聯(lián)系方式

郵箱：yuweizhu@hit.edu.cn  

個(gè)人簡(jiǎn)介

2005-2009年：遼寧大學(xué) 生物化學(xué)與分子生物學(xué)學(xué)士

2009-2014年：中國科學(xué)技術(shù)大學(xué) 生物化學(xué)與分子生物學(xué)博士

2017-2019年：哈爾濱工業(yè)大學(xué) 生物醫學(xué)工程學(xué)博士后

2020年-：哈爾濱工業(yè)大學(xué) 生物化學(xué)與分子生物學(xué)副教授

研究方向和領(lǐng)域

CRISPR-Cas system and Anti-CRISPR proteins

研究內容

My focus is on determining the structures of DNA/RNA-protein complexes using X-ray diffraction and Cryo-EM.

通過(guò)X-射線(xiàn)晶體學(xué)及冷凍電鏡等結構生物學(xué)手段解析蛋白質(zhì)與核酸復合物的結構

研究成果

1. Zhang, K^?., Wang, S^?., Li, S^?., Zhu, Y^?., Pintilie, GD., Mou, T., Schmid, MF., Huang, Z., Chiu W. Inhibition mechanisms of AcrF9, AcrF8, and AcrF6 against type I-F CRISPR-Cas complex revealed by cryo-EM. Proc Natl Acad Sci U S A, 2020 Mar 13. (Co-first author)

2. Dong, D^?., Zheng, L^?., Lin, J^?., Zhang, B., Zhu, Y., Li, N., Xie, S., Wang, Y., Gao, N., Huang, Z. Structural basis of assembly of the human T cell receptor-CD3 complex. Nature, 2019 Sep;573(7775):546-552.

3. Zhu, Y., Huang, Z. Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools. National Science Review, Volume 6, Issue 3, May 2019, Pages 438–451.

4. Dong, L^?., Guan, X^?., Li, N^?., Zhang, F., Zhu, Y., Ren, K., Yu, L., Zhou, F., Han, Z., Gao, N., Huang, Z. An anti-CRISPR protein disables type V Cas12a by acetylation. Nat Struct Mol Biol. 2019 Apr;26(4):308-314.

5. Guo, M^?., Ren, K^?., Zhu, Y^?., Tang, Z., Wang, Y., Zhang, B., Huang, Z. Structural insights into a high fidelity variant of SpCas9. Cell Res. 2019 Mar;29(3):183-192.(Co-first author)

6. Zhu, Y., Zhang, F., Huang, Z. Structural insights into the inactivation of CRISPR-Cas systems by diverse anti-CRISPR proteins. BMC biology, 2018 Mar 19;16(1):32.

7. Dong, D^?., Guo, M^?., Wang, S^?., Zhu, Y., Wang, S., Xiong, Z., Yang, J., Xu, Z., Huang, Z. Structural basis of CRISPR-SpyCas9 inhibition by an anti-CRISPR protein. Nature, 2017 Jun 15;546(7658):436-439.

8. Wu, D^?., Guan, X^?., Zhu, Y^?., Ren, K., Huang, Z. Structural basis of stringent PAM recognition by CRISPR-C2c1 in complex with sgRNA. Cell Res, 2017 May;27(5):705-708. (Co-first author)